An established laboratory in the Department of Pathology, Microbiology and Immunology is seeking an RAI with the desired qualification/experience:
- Animal handling and colony management in a biology, immunology, and/or molecular biology research laboratory
- Desire to learn one or more of the following new techniques:
tissue culture techniques: sterile methods and related methods: flow cytometry and flow sorting
molecular biology techniques: elementary DNA isolation, cloning, gene transfer and related methods; elementary protein techniques such as recombinant protein expression in one or more systems (bacteria, yeast, insect cells, mammalian cells), immunoprecipitation, Western blotting, and related techniques
protein chemistry techniques: affinity chromatography, liquid chromatography, and other specialized methods used in our laboratory
- Some experience in a biology, immunology, and/or molecular biology laboratory is desirable
This Research Assistant I (RA-I) will have the primary responsibility of handling and maintaining our mouse colonies consisting of inbred and genetically altered knockout and transgenic mouse strains. This primary responsibility will entail tail DNA genotyping, setting up breeding to obtain desired animals of specific genetic background, and other related activities.
Additional job responsibilities of this RA-I position will involve assisting laboratory members--i.e., the PI, students, fellows, research faculty, & staff scientists, with experiments under supervision.
The Department of Pathology, Microbiology and Immunology at Vanderbilt has a long and rich history of excellence in teaching, clinical service, and research. Our collaborative efforts in personalized medicine in cancer, pharmacogenomics and the creation of Diagnostic Management Teams are at the "leading edge" of a revolution in the practice of pathology. The department lives and breathes our mantra that we are a "laboratory of the whole" for defining the future of pathology. We are committed to taking a leadership role in both basic and translational research in these areas of focus.
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- Quickly learn handling, breeding, and genotyping of mice
- Quickly learn mouse colony management and record keeping
- Understand and follow experimental protocols for tail DNA genotyping, setting up breeding, tissue culture, and others as needed
- Ensure mouse line/s are generated in a timely fashion and available for experiments
- Record, compile, interpret, and communicate results from mouse genotyping appropriately
- Provide appropriate written documentation of mouse genotypes
- Follow University safety protocols as the work in the laboratory may involve the use of combustibles, radioactivity, toxic material, and/or carcinogens
- Prepare buffers and reagents as needed; this will require calculating molarity, and serial dilutions, and other similar tasks
- Read all equipment manuals; use them according to manufacturer instructions
- Prepare, operate, and troubleshoot equipment used in experimental procedures
- Maintain and repair equipment according to manufacturer and University guidelines
- Maintain sterile conditions as required in a BioSafety Level (BSL)-2 laboratory
- Demonstrate an understanding of acceptable procedures and techniques
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period. Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As t...he largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.